Does the solvent used during making a standard curve for uv-vis spec need to be the same as one used for actual entrapment/loading studies?

I am studying entrapment of a Cox-2 inhibitor in solid lipid nano-particles (SLNs). While making a standard curve, I used methanol-water mixture as a solvent (as the drug is sparingly water...

03 March 2013 1,097 3 View