Anup Chugani

3 Questions 2 Answers 0 Followers

Questions related from Anup Chugani

When is the best time to record the SYBR signal? After annealing or after extension?

I was wondering if there is any related literature as to why we acquire florescence data after Annealing step in qPCR. Ofcourse, there is also a possibility of acquisition after Extension but, how...

06 June 2015 3,837 0 View

Can human recombinant BDNF (commercially available) be used for stimulation in rats and mice?

Commercially available BDNF is human recombinant protein. Can you use it for stimulating primary neuronal cultures of mice and rats? If yes, what is the basis of this cross species activity?

06 June 2013 2,484 1 View

What concentration of cDNA to be used for qPCR for miRNA quantitation?

I am using Taqman microRNA assay to quantify miRNAs. Do you have to quantify the cDNA before going for qRT-PCR? The initial concentration of total RNA is 10ng/15uL reaction. What would be to...

05 May 2013 6,696 3 View