Antonio S Minero

6 Questions 7 Answers 0 Followers

Questions related from Antonio S Minero

Salmon sperm DNA immobilization to 96-well micro-titer plate?

Hi, does anybody know a good protocol for "non-specific" immobilization of salmon sperm DNA onto glass bottom of microtiter plates? Thanks

22 November 2021 3,733 0 View

Do magnesium pyrophosphates form in vivo?

It is well known that Mg ions bind PPi moieties (pyrophosphates) during DNA amplification such as PCR, LAMP or RCA. I assume they should do the same during DNA replication in vivo, i.e. inside...

14 September 2021 4,361 1 View

What is molecular weight of Dengue virus (Serotype 2)

Hi all, I need to convert mg/ml concentration into particles/ml or, ideally, molarity. Does anyone know molecular weight of a Dengue viral particle (with all structural proteins)?

19 July 2017 5,522 1 View

How can I extract exlusively 16S rRNA from E.Coli or how to quantify the 16S rRNA "weight" in the whole nucleic acid content extracted from E.Coli?

Hi, I would like to detect E.Coli after amplification of 16S rRNA (I am talking about part of the sequence, i.e. 230 nt). So I let cells to grow and then use Qiagen DNA/RNA extraction kit. Then I...

25 May 2016 2,373 1 View

Magnesium pyrophosphates and DNA

Hi, I have recently addressed topic of magnesium pyrophosphates binding to DNA during amplification, and that these polycationic clusters stabilize short hybrids of DNA through electrostatic...

01 January 1970 7,427 0 View

Triplex DNA "switching"

Have you noticed, formation and dissociation of triplex DNA is not fully reversible upon pH titration? After several cycles it becomes almost irreversible, i.e. fold in triplex conformation. Any...

01 January 1970 4,015 1 View