Anthony Harrington

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Questions related from Anthony Harrington

Can anyone help with purifying crude protein extracts?

Hi, I have a question about crude protein lysates (extracts). A fellow lab member in my lab is trying to isolate a 27KDa protein from here crude protein lysate. She mentioned that this is the...

07 July 2014 1,409 14 View

How can I desalt a crude extract for LC-MS analysis?

I am in the process of sending some samples for LC-ESI-MS analysis and was curious about removing any salts that might be in my extracts. The samples I am sending out are...

07 July 2014 6,352 4 View

Can I anybody explain to me why we use a 1:1:2 ratio of amino acid, uronium coupling reagent, and DIEA for Fmoc SPPS?

I have been using a 1:1:1 ratio of amino acid, coupling reagent, and DIEA but from the papers and books that I have read on Fmoc SPPS, I see most use a 1:1:2 ratio. What is the purpose of using 2...

01 January 1970 2,699 0 View