Anterpreet Chahal

3 Questions 12 Answers 0 Followers

Questions related from Anterpreet Chahal

What is the best way to calculate ROS generation in cell culture when done with DCFDA probe and flourescence is measured with flourimetry ?

I am currently checking ROS generation in human cells with DCFDA dye and measuring fluorescence on fluorimetry . What is the best way to express results? What calculations need to be done to...

04 April 2017 6,273 3 View

Which is the best column for preparative HPLC for purification of compounds from plant extracts? Is there any specific range for flow rate ?

I want to purify compounds from extract of Emblica officinalis . I want to know about the columns used and what flow rate is required for eluting out the natural compounds ? Also, I want to know...

10 October 2016 8,199 7 View

Can anyone please suggest a solvent system for TLC for eluting compounds from ethyl acetate fraction of Emblica officinalis (Amla) ?

I am working on crude methanolic extract of Amla which is further fractionated into chloroform, ethyl acetate , butanol fractions . I have got best respective activities in ethyl acetate extract ....

07 July 2016 1,391 3 View