Antara Biswas

3 Questions 1 Answers 0 Followers

Questions related from Antara Biswas

What is the minimum number of cells required for fluorescence in situ hybridization?

I have to perform a FISH experiment with limited number of cells and I am trying to estimate the minimum number of cells required to do FISH. Cells are from mammalian cell line. I failed in doing...

13 January 2021 8,022 1 View

Single-cell cloning from SkBr3?

Did anyone tried single-cell cloning from SkBr3 cell line. I am failing in growing cells post single cell seeding in wells. Any help is appreciated

28 September 2020 8,914 4 View

Why can I purify GST protein from vector transformed DH5α cells, but can't purify GST tagged protein of interest?

Attempting affinity purification. Vector backbone is pGEX-6P1. Insert encoding my protein of interest is 2.4 kb in length. Composition of lysis/binding buffer is 20 mM Tris-HCl (pH 8.0), 500 mM...

12 June 2014 9,824 1 View