Annie Newell-Fugate

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Questions related from Annie Newell-Fugate

3T3-L1 cells--media pH shifting! What to do? Help!?

We have a 3t3l1 cell line---never had problems before but all of the sudden having issues with phd of media going off. Using same base media on primary rodent preadipocytes with no issues...media...

01 January 2016 5,493 7 View

Does anyone have input on the best way to extract protein from white adipose tissue?

I have tried homogenization, followed by centrifugation at high speeds for 40 minutes, followed by centrifugation through a concentrator filter. I suspect my BCA assay was not accurate as I had...

01 January 2015 1,802 5 View

I need to transport a lot of frozen tissue samples (~ 24 hour transit). How much dry ice per lb of samples should I pack in coolers?

I will be driving these in the back of a U haul truck across several states. It is imperative that the samples stay frozen until they can be put into a -80 freezer. On line I have read 10 lbs of...

01 January 2015 5,123 6 View

Has anyone had success with ceiling culture of mature adipocytes from primary tissue?

I have tried the Zhang et al 2000 protocol with flasks but have had only stromal cell looking things attach...

05 May 2013 6,303 3 View