Annette E. Sköld

5 Questions 4 Answers 0 Followers

Questions related from Annette E. Sköld

Does regularly running CST beads improve the performance of the flow cytometer?

In my lab, we are only a few ppl running multicolor panels in a 14 color BD Fortessa. Most users only use 1 color and therefor does not run CST beads. My question is if it's recommended to run the...

01 June 2017 3,279 3 View

Shelf life of TRIS?

I just started in a new lab and am about to start running western blot. However, the TRIS powder they have here is from 2002 and I'm a bit reluctant using it. Does anyone have experience using old...

27 April 2016 6,521 5 View

How long in advance can I make an Agarose gel before running it?

I'm having a busy lab day, and am considering to make my gel either the day before or in the morning the same day, and then run the gel in the evening. Is this ok? I'm studying the stability of...

29 September 2015 9,699 8 View

Does anyone know of an efficient, low-toxic caspase-1 inhibitor for in vitro cell culture?

I would like to inhibit the inflammasome in my cultured dendritic cells, but am a bit unsure of which one to buy. Does anyone have any recommendations? Thanks!

19 August 2013 2,913 2 View

Is mRNA immunostimulatory?

If mRNA would get into the endosomes of human cells would it be recognized by TLR7/8? And if it would be recognized, would it be immunostimulatory, or does the 5' cap and 3' poly A-tail prevent...

14 February 2013 2,821 9 View