6 Questions 5 Answers 0 Followers
Questions related from Annette E. Sköld
In my lab, we are only a few ppl running multicolor panels in a 14 color BD Fortessa. Most users only use 1 color and therefor does not run CST beads. My question is if it's recommended to run the...
01 June 2017 3,340 3 View
Hi, I'm planning to do a migration experiment where A549 cells are migrating into a Matrigel or similar. Most protocols use microscopy as a read-out method, but I wonder if it's possible to melt...
21 July 2016 1,787 5 View
I just started in a new lab and am about to start running western blot. However, the TRIS powder they have here is from 2002 and I'm a bit reluctant using it. Does anyone have experience using old...
27 April 2016 6,576 5 View
I'm having a busy lab day, and am considering to make my gel either the day before or in the morning the same day, and then run the gel in the evening. Is this ok? I'm studying the stability of...
29 September 2015 9,757 8 View
I would like to inhibit the inflammasome in my cultured dendritic cells, but am a bit unsure of which one to buy. Does anyone have any recommendations? Thanks!
19 August 2013 2,966 2 View
If mRNA would get into the endosomes of human cells would it be recognized by TLR7/8? And if it would be recognized, would it be immunostimulatory, or does the 5' cap and 3' poly A-tail prevent...
14 February 2013 2,872 9 View
