Can I adapt primers used for 250 bp amplicon sequencing into primers for 500 bp amplicon Illumina sequencing on MiSeq?

Untill now I have always used the Illumina technique for sequencing the V4 region of the 16S rRNA on the Miseq System. Primers targeting the 16S V4 region were designed and tailed with sequences...

19 November 2014 4,332 4 View