Anne Marie (Mulligan Tuttle) Gannon

6 Questions 19 Answers 0 Followers

Questions related from Anne Marie (Mulligan Tuttle) Gannon

Can you use deuterated testosterone as an internal standard for ELISA?

Looking to get away from using C14 spike-in as the standard and was wondering if using deuterated testosterone is a viable option for testosterone ELISAs. Thank you!

06 June 2016 2,536 11 View

How much yield (ng/ul) do you get with RNA extraction from serum?

Just wondering what I should expect in terms of a yield in ng/ul when isolating RNA using the Qiagen miRNeasy kit from serum. Thanks!

01 January 2015 5,971 2 View

Implantation site detection method - can anyone help?

I need to stain rodent implantation sites but then want to use the uteri for histological fixation. I understand that the use of ammonium sulfide renders the tissue unusable for fixation. Any...

06 June 2013 3,076 13 View

Can anyone recommend a good protocol for C. elegans spike-in for miRNA from serum?

I'm looking for a good protocol for spike-in for rat serum miRNA quantification. I've heard that the C.elegans spike-in works well, but don't have an appropriate protocol for it. Any tips would be...

04 April 2013 8,977 10 View

Does the volume of p-npp for alkaline phosphatase make a huge difference in a sandwich ELISA?

I am running a sandwich ELISA and realised far too late that I have run low on SigmaFast p-npp tablets for my detection step. In the product information insert, Sigma suggests 200 microlitres of...

07 July 2012 1,393 6 View

Using serum as sample in Western blot

Has anyone ever successfully used mouse serum as the sample in a Western blot? If so, what (if anything) did you use to dilute it?

05 May 2012 9,052 2 View