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Questions related from Anne Marie (Mulligan Tuttle) Gannon
Looking to get away from using C14 spike-in as the standard and was wondering if using deuterated testosterone is a viable option for testosterone ELISAs. Thank you!
06 June 2016 2,540 11 View
Just wondering what I should expect in terms of a yield in ng/ul when isolating RNA using the Qiagen miRNeasy kit from serum. Thanks!
01 January 2015 5,975 2 View
I need to stain rodent implantation sites but then want to use the uteri for histological fixation. I understand that the use of ammonium sulfide renders the tissue unusable for fixation. Any...
06 June 2013 3,079 13 View
I'm looking for a good protocol for spike-in for rat serum miRNA quantification. I've heard that the C.elegans spike-in works well, but don't have an appropriate protocol for it. Any tips would be...
04 April 2013 8,980 10 View
I am running a sandwich ELISA and realised far too late that I have run low on SigmaFast p-npp tablets for my detection step. In the product information insert, Sigma suggests 200 microlitres of...
07 July 2012 1,396 6 View
Has anyone ever successfully used mouse serum as the sample in a Western blot? If so, what (if anything) did you use to dilute it?
05 May 2012 9,055 2 View