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Hi all, I have a set of cell culture samples embedded in 1% agarose. They have been cultured for 14 days. They have been fixed with 4% PFA. I am wondering if I can separate the whole cells from...
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Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...
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Last year, we got HUVEC (RFP) and we were having trouble since Day 1 (it has proven to be a lot more work than I expected compared to other cells. After we gotten the cells, we thawed them at 38C...
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Hello, I am interested in this research field. I have the prototype of my microfluidic chips, but I am having a hard time finding literature that says exactly how they incubate their chips (with...
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Hello, I have been doing some literature research on direct PDMS 3D printing on microchips. As far as I know, researchers have been printing a master mold, then cast the PDMS resin and cure it to...
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