Less Luciferase signal when cells transfected with more DNA does it make sense?

Hi all, I have a following problem. I have cloned a promoter of interest at 5´of Luc2 sequence in pGL4.10 vector (no minimal promoter, no enhancer). I transfect HEK293 cells with 0.5 or 1 ug of...

11 November 2016 608 4 View