Anna P Stephan

3 Questions 2 Answers 0 Followers

Questions related from Anna P Stephan

Issues with ECL or antibody leading to uneven exposure of western blot?

Hi all, Recently I have been having trouble exposing my tubulin. There seems to be an uneven exposure of the lanes, yet my ponceau looks even across. From what I have read online, this could...

16 September 2024 8,545 3 View

Has anyone used MyoD as a driver for muscle specific knockdown of a gene and observed a change in beta-actin or tubulin levels?

I am wondering if anyone has used MyoD as a driver in mice (to knockdown a gene in muscle specifically) and has extracted protein, run a western blot and noticed in their KO group (when comparing...

28 April 2023 7,692 1 View

Housekeeper for unheated western blot samples?

I am using a mitochondrial cocktail antibody on mouse muscle samples in western blot. Abcam suggested to use unheated samples as the detection of some of the complexes is sensitive to heating. Has...

28 December 2022 9,262 6 View