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Questions related from Anna Parker
I am studying a post translational modification through Co-IP and I would like to perform a denatured IP to confirm that my results are not from post translational modification of a different...
13 March 2017 6,912 2 View
I am considering trying to elute my immunoprecipitation by boiling in loading buffer. I was wondering if the remaining eluate I don't run can be saved and used in another western at a later date?...
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I am working with plasmids containing aion channel, with the goal of eventually using them for transfection in Hek cells. The problem I am having is preparing these plasmids at the bacterial...
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I ligated an approximately 2kb (restriction digested and purified) insert into a 5.3kb vector (restriction digested and dephosphorylated) using a T4 ligase. I used varying insert:vector ratios...
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I am having trouble with my tris-glycine running buffer. It's very cloudy, I would guess because the SDS has come out of solution. I don't remember having this problem in the past, and I have...
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