Anna Lara Ernst

3 Questions 9 Answers 0 Followers

Questions related from Anna Lara Ernst

Why is fungal/bacterial DNA isolated with Qiagen Powerlyzer Powersoil Kit not stable?

We have isolated DNA with the Qiagen Powerlyzer Powersoil Kit (with addition of Proteinase K) for NGS downstream applications (16SrRNA/ITS2) and encountered the problem that the isolated DNA is...

27 October 2020 7,557 2 View

Less DNA than RNA in PCR with same nucleic acid input

We were running PCR for a 450bp bacterial V1 and V2 region product with each 30ng of either cDNA or DNA. But the bands in the gel appeared stronger for the cDNA (as in RNA) and the DNA was very...

01 January 1970 1,216 3 View

Unhappy smiley face shaped bands in gel?

I am running PCRs with cDNA and DNA followed by agarose gel electrophoresis (1.5% gel, 1x TAE buffer, 3µl PCR product loaded (5µl PCR product with 1µl 6x loading dye (thermofisher), 80V for 1,5...

01 January 1970 8,491 20 View