Anna Aiken

4 Questions 4 Answers 0 Followers

Questions related from Anna Aiken

Help with drying 6M HCl from sample after amino acid hydrolysis?

I have digested plant samples with 1 mL of 6M HCl. I am now attempting to dry the samples in a vacuum oven. I have it set at 60 deg 6, 60 mmHg and after 3-4 hours I am not seeing a reduction in...

28 December 2018 3,969 2 View

How can I clean up my plant sample before hydrolysis for amino acid analysis?

Would it be appropriate to do an extraction with a non-polar solvent system such as chloroform and hexane (to get rid of oils, pigments, etc) before hydrolyzing my plant samples for AA analysis? I...

11 December 2018 7,978 5 View

How should I remove excess HCl from sample hydrolysate before HPLC?

I have digested my plant sample (50 mg) in 1 mL of 6N HCl for amino acid analysis. How should I proceed to dry the sample? I have a freeze dryer but no acid trap so I cant lyophilize. Please advise!

04 December 2018 2,238 5 View

Black precipitate after OPA derivatization of hydrolysed plant protein extract?

i am attempting amino acid analysis of leaf protein extracts. Upon addition of 5uL OPA reagent to 5ul of extract a black precipitate forms. This precipitate appears to have bound early eluting...

29 April 2018 9,632 3 View