Ann-Kristin Diederich

3 Questions 3 Answers 0 Followers

Questions related from Ann-Kristin Diederich

Bacterial RNA of stationary phase produces very distinct CT?

I am quite new to real time PCR, so maybe I don't see my fault. I isolated RNA of bacterial cell culture of stationary phase and transcripted it to cDNA. The RNA looks fine on the gel. When I do...

21 October 2013 4,871 1 View

I want to do 2D preparative thin layer chromatography and stain with iod for detection. How likely is the probability that my lipids will oxidize?

For development I use a mixture of CHCl3, MeOH and H2O.

26 February 2013 1,309 2 View

Does Coomassie stain substances with just one or few rather primary amino groups?

Substance is stainable with Ninhydrin, but does not appear on SDS when stained with very sensitive coomassie staining.

07 February 2013 5,193 3 View