Ankita Bane

4 Questions 8 Answers 0 Followers

Questions related from Ankita Bane

How do I conclude if my gene has low expression based on the results obtained?

Hello all, I am working on genes affected in cholesterol metabolism in patients with Coronary Artery Disease and PCSK9 is one such target gene in my study. I have managed to obtain stable results...

14 June 2020 7,188 9 View

How do I interpret drastic differences in Ct values between healthy controls and disease controls?

I am currently validating 18S rRNA, HPRT1 and GAPDH as housekeeping genes to normalize data obtained from my case:control qPCR study using human peripheral blood. Group 1 - Patients (cases)...

22 July 2019 607 3 View

Does anyone have experience with isolating mRNA from frozen whole blood samples stored in RNAlater?

I have frozen blood samples - preserved in RNAlater stored at - 80. I'm planning to use the Ambion Ribopure blood kit or Trizol for RNA isolation. Can anyone provide me a protocol for removal of...

30 August 2018 8,844 4 View

How do I compare plasma protein levels (ng/ul) vs mRNA expression (whole blood/qPCR) for a target molecule for the same set of patients?

I have quantified plasma protein (ELISA) and mRNA (whole blood; RT-PCR) for a candidate molecule. Independently, a comparison of plasma levels between 2 groups shows a significant difference;...

01 January 1970 4,296 3 View