Ankit Sabharwal

3 Questions 2 Answers 0 Followers

Questions related from Ankit Sabharwal

How do I estimate protein concentration when extracting ATP using TCA in tissue sample?

I am trying to estimate ATP in tissue sample. I homogenize the sample in 0.5% TCA and neutralize the supernatant (gentle mixing on ice) with 1 M Tris-acetate buffer to dilute the TCA to 0.05%. I...

22 January 2016 3,974 0 View

How does the pH of the mitochondria isolation buffer affect the mitochondrial isolation?

If the pH of the buffer is around 6-6.5, does it affects the mitochondrial isolation. The cells are lysed and are pelleted out at 600g. But in next step, when supernatant (containing cytosolic...

20 March 2015 3,255 4 View

How can I stain mitochondria in fixed cells or fixed zebrafish embryos?

I wish to use a method that doesn't employ any fluorescent stain like FITC or fluorescein because my probe is GFP tagged. Thanks

08 March 2015 4,392 13 View