Ankit Sabharwal

2 Questions 2 Answers 0 Followers

Questions related from Ankit Sabharwal

How does the pH of the mitochondria isolation buffer affect the mitochondrial isolation?

If the pH of the buffer is around 6-6.5, does it affects the mitochondrial isolation. The cells are lysed and are pelleted out at 600g. But in next step, when supernatant (containing cytosolic...

20 March 2015 3,240 4 View

How can I stain mitochondria in fixed cells or fixed zebrafish embryos?

I wish to use a method that doesn't employ any fluorescent stain like FITC or fluorescein because my probe is GFP tagged. Thanks

08 March 2015 4,377 13 View