While doing cytochrome C standard curve preparation, brown colored cytochrome C changed to light brown instead of blue. can anyone tell me why?

the assay was done using TMBZ solution which was made by dissolving 0.01 g of TMBZ in 5 ml of methanol and 15 ml of 0.25 M sodium acetate buffer (mixing sodium acetate and glacial acetic acid) (pH...

09 September 2016 695 2 View