Anjali Patni

3 Questions 16 Answers 0 Followers

Questions related from Anjali Patni

Related to the protein precipitation and its re-suspension in a suitable buffer?

I'm facing a problem with respect to the protein loss after acetone precipitation, followed by its insolubility in the 100mM Ammonium bicarbonate solution, I also tried dissolving the protein...

03 May 2018 1,187 5 View

How can we carry out data filtering or data refining after obtaining raw mascot data?

I've obtained the mascot protein list in mgf file And I need to carry out further identification and validation steps My data comprises of treated, untreated and control and I need to determine...

15 February 2018 9,493 8 View

Can we perform In gel or In solution digestion using Chymotrypsin or Proteinase K just like with Trypsin?

If yes then will it give the same Protein coverage just like we get using Trypsin?

07 November 2017 6,776 4 View