Anjali Patni

4 Questions 16 Answers 0 Followers

Questions related from Anjali Patni

Why reduction and alkylation done prior to precipitation step in kit based digestion protocol?

This kit based protocol says about reduction and alkylation of protein prior to acetone precipitation step, Reduction and alkylation basically leads to unfolding of the protein chain. And acetone...

28 May 2018 1,277 0 View

Related to the protein precipitation and its re-suspension in a suitable buffer?

I'm facing a problem with respect to the protein loss after acetone precipitation, followed by its insolubility in the 100mM Ammonium bicarbonate solution, I also tried dissolving the protein...

03 May 2018 1,237 5 View

How can we carry out data filtering or data refining after obtaining raw mascot data?

I've obtained the mascot protein list in mgf file And I need to carry out further identification and validation steps My data comprises of treated, untreated and control and I need to determine...

15 February 2018 9,552 8 View

Can we perform In gel or In solution digestion using Chymotrypsin or Proteinase K just like with Trypsin?

If yes then will it give the same Protein coverage just like we get using Trypsin?

07 November 2017 6,839 4 View