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Dear all, I am want to generate a k.o. with CRISPR/CAS9 in H292 cells and have issues with the transfections. It does not matte if its for plasmid or RNP format. Does anyone have a working...
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I am trying to transfect HFL-1 cells with a plasmid DNA. I used Lipofectamine 3000 from ThermoScientific, however the cells seem to react quite sensitive to the transfection (tried different...
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