Anita Brozkova

3 Questions 2 Answers 0 Followers

Questions related from Anita Brozkova

What can be the reason we can not detect BRCA1 and BRCA2 proteins using immunoblotting?

Hi there, I am using an optimalized protocol to detect BRCA1 and BRCA2 by Western blotting. This protocol worked for me nicely with one protein extract prepared from the whole 10 ml flask of U2OS...

04 April 2016 4,857 5 View

ChIP troubles - do you know, what can cause extremely high input Ct values?

I have continuous problem with my ChIP - I am using Dynalbeads and following protocol, that has been used in my lab and worked fine. I am working with budding yeast cells, my input values (Input...

02 February 2015 4,861 1 View

Do you have experience with a Rad53 antibody from Abcam S. cerevisiae?

We bought this antibody: Anti-Rad53 antibody (ab104232) to investigate a checkpoint activation in yeast. On Western we saw two very sharp and saturated unspecific bands besides Rad53 the first one...

12 December 2014 1,326 1 View