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Questions related from Anirudh Gautam M
I have been trying to generate clones using pENTR DTOPO for gateway cloning. I am able to amplify my insert, set up a reaction and get colonies. But when I do a colony PCR with insert specific...
10 October 2014 9,414 8 View
My template amplifies when I use Hi fidelity kapa and not when I use Taq polymerase (normal/regular) . Is there any limitation to template size for taq polymerase? I had given it 1 minute and 45...
07 July 2014 8,670 16 View
I am working with a protein which becomes soluble thanks to MBP. I do not see much expression with GST and also see the contamination of GroEL when I overexpress with GST. However the protein...
07 July 2014 9,129 8 View
My lab has a centricon with a minimum of 3 kda cut of . I would like to concentrate a 1 kda peptide. Any suggestions?
07 July 2014 7,188 25 View
