Anil Gaikwad

4 Questions 7 Answers 0 Followers

Questions related from Anil Gaikwad

Can anyone tell me how to distinguish between cDNA and reverse transcribed RNA of the same?

We are trying to standardize eSiRNA method, but it is difficult oto distinguish between cDNA and reverse transcribed RNA, can anyone tell me how to be sure about reverse transcription (except...

16 June 2015 832 3 View

Can you suggest a protocol for a differentiation of Mesenchymal stem cells into brown adipocytes?

There are many protocol on the internet with different chemicals used for differentiation induction. Can you provide components of differentiation induction media?

30 January 2015 3,256 3 View

Has anybody experienced B-cell receptor signaling activation in anything other than B-cells, especially in case of inflammation?

Like liver, muscle cell, kidney or other cell types? If yes, then what is relevance of BCR signaling activation in other cell types?

27 June 2014 6,525 3 View

What is easy way of concentrating lentivirus particles and is there any way to titrate lentiviral particles without kit?

We are trying to concentrate lentiviral particles after lentiviral production but we do not see pellet of particle when centrifuged at 15000 rpm over the day (7-7.5 hrs). Do we get to see pellet?...

21 January 2014 5,330 22 View