8 Questions 13 Answers 0 Followers
Questions related from Anette Martensson
I am setting up a direct ELISA and have a standard curve which I fit to a 5-PL curve. When I try to calculate the concentrations of the points in the standard curve using the obtained 5-PL curve I...
13 March 2018 1,461 2 View
I am looking for a good software for fitting curves and would like to hear what software other people recommend. I want to be able to fit 4- and 5- parameter logistic curves and linear regression....
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I am wondering what your opinion is on what cell density is optimal for expression of antibodies by transient transfection of suspension adapted HEK-293 cells in batch culture using PEI as...
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I am trying to concentrate a protein using Amicon Ultra centrifugal device. I have done this several times before without any problems. This time I had a larger volume of my sample so I had to...
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I want to set up an assay to detect internalization of an antibody in HEK cells. I want the assay to be ELISA based since I don't have access to a FACS machine or a confocal microscope. Does...
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I am purifying a protein from a cell supernatant with a His-trap column from GE. I get quite low yield and it seems as if the protein doesn't bind very well since I can obtain protein when...
06 September 2012 4,780 43 View
I want to purify IgY from eggs using a PEG based protocol since this is cheap and fairly simple, compared to amoniumsulphate methods. I have only found protocols where one has to centrifuge at...
01 August 2012 1,247 7 View
I am trying to tranfect CHO cells with the murine FcgammaRIII. I cotransfect it with the murine gamma chain as I have read that this is required for protein expression. I have tried lipofectamine,...
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