6 Questions 12 Answers 0 Followers
Questions related from Andrew Whiteoak
To begin, I do know that Tm = 4(G+C)+2(A+T) So I don't need that to be reiterated. what I wonder is whether wobbles e.g. R = A/G Y = C/T K = G/T M = A/C W = A/T S = C/G B = C/G/T D = A/G/T H =...
10 October 2013 6,987 4 View
I would imagine an equation must, or should, exist which takes in the parameters of primer length, base type proportions and wobble types to produce a relative specificity gradient between various...
10 October 2013 2,573 16 View
I am designing primers and would like to retain as much specificity as possible. However, due to variation in the gene I am coming across many issues where wobbles would need to intervene, and...
10 October 2013 490 16 View
I know that V=IR where V is voltage, R is resistance and I is the current in mA; so my problem is that the electrophoresis units available to me allow fellow researchers to change these parameters...
10 October 2013 7,496 5 View
Once I have found sequences of a particular gene for animals of a related species, and have produced an alignment (I used Clustal Omega,) how do I then format this data to be used on Primer...
09 September 2013 8,524 6 View
A paper I've read says, "The melting temperature for primer regions should be 60-65c in the case of GC rich and 55-60c in the case of AT rich" (for LAMP sequencing.) I do not know how to predict...
09 September 2013 5,788 16 View
