Andrew John Hollins

3 Questions 14 Answers 0 Followers

Questions related from Andrew John Hollins

Can anyone recommend ARMS PCR primers for KRAS mutational analysis?

I have come across ARMS PCR for 'simple' mutation analysis, is it as straight-forward as the schematic diagrams would have you believe? What are the considerations that are key to look out for? If...

12 December 2014 1,524 2 View

What are the best medium/conditions for serum free production and harvest of recombinant protein from a HEK293 line?

There appear to be a number of media on the market for serum free culture of HEK293 lines, but I am after some feedback of what might work most efficiently and any pitfalls to avoid. As with all...

06 June 2013 6,557 1 View

I have been shown an apparent Mycoplasma infection of mES cultures that appear motile (spinning / jiggling) if not mycoplasma what could it be?

We have looked at the objects under x40 obj mag and they appear to be around 1µm in size (comparing to an appropriate graticule). They are small dark, and spin / jiggling with varying speeds. The...

11 November 2012 3,623 8 View