Andrew Coleman

3 Questions 8 Answers 0 Followers

Questions related from Andrew Coleman

Why a lack of Cre-mediated recombination following nucleofection of post-natal mouse cortical neurons?

Hi all, I am trying to delete a protein of interest from postnatal cortical neuron cultures from floxed mice by introducting Cre via nucleofection (AMAXA system), but fail to see any loss of...

09 September 2018 7,640 2 View

Which commercially available N-terminal GluA1 antibodies work best for surface staining in dissociated neurons?

I'm trying to detect surface GluA1 levels in dissociated neurons both at steady state and after a chemical LTP paradigm. It seems like many labs use a Millipore antibody (MAB2263/RH95) on...

12 December 2017 6,439 0 View

How to optimize dual transfection of primary hippocampal neurons via lipofectamine?

I am trying to transfect primary rat hippocampal neurons with two constructs - one encoding a soluble fluorescent protein and the other tagged protein of interest. In transfected cells I always...

05 May 2016 2,213 7 View