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Questions related from Andrew Conway Morris
I'd like to image the nuclear envelope in live primary human neutrophils and was wondering if anyone had any techniques for doing this. Neutrophils are short-lived cells which largely precludes...
04 July 2018 6,332 0 View
We are stimulating human neutrophils with priming agents and chemoattractants such as C5a, with the neutrophils suspended in 1% serum and RPMI with HEPES. This is being conducted in a standard...
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I've been attempting to extract Tregs from healthy human blood, so far using percol gradients to obtain PBMCs and then CD4+CD25+CD127lo beads/columns from MACS I've managed to get about 100,000...
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