Andrés Castillo

4 Questions 1 Answers 0 Followers

Questions related from Andrés Castillo

Agrobacterium selection methods?

Good morning, We are trying to select our Agrobacterium tumefaciens colony with X-gal and IPTG assay since our plasmid has LacZalpha promoter. We cannot select the colonies by antibiotic selection...

02 February 2022 5,090 2 View

Streptomycin for agrobacterium tumefaciens GV3101 transformation?

I have GV3101 Agrobacterium Tumefaciens strain with Strep resistance. I want to transform them with a plasmid with the same resistance. Can I use this strain for the plasmid transformation with...

14 September 2021 8,849 2 View

Does the agroinfiltration have to be done inmediately after the agrobacterium transformation?

Hi everyone, I would like to know if the agrobacterium cells can be stored after their transformation with the plasmid of interest for future plant transfection via agroinfiltration. If the answer...

27 August 2021 5,417 3 View

Strange sgRNA scaffold in Addgene plasmids?

Sorry for my ignorance, but I am having difficulties to understand why in the plasmid pHSN6A01 (https://www.addgene.org/browse/sequence/258465/), between the pol III promoter (AtU6-26) and the...

29 June 2021 2,409 1 View