Andrei Moroz

4 Questions 18 Answers 0 Followers

Questions related from Andrei Moroz

Why is my mAb recognizing multiple bands in blott (attached image)?

We produced mAbs at my lab (classical 1975 hybridoma tech). One of my clones produced one mAb that was promising. When we constructed it, we used subtractive immunization, which consists of...

02 February 2019 3,755 8 View

Has anyone used flow cytometry (on 96 well microplates) during the screening of mAbs? How does it work? Which cytometers can receive the plates?

03 March 2018 4,671 2 View

What is the best marker to distinguish epithelial and mesenchymal cells growing together in vitro?

Although very basic, we need help: We have tried to isolate epithelial cells from cornea, however, we have in our culture dishes two different cell populations (morphologically speaking):...

10 October 2013 4,088 7 View

What is the best way to start to screen for monoclonal antibody cytotoxicity effects on tumoral cell lines?

I need to know which is the right scale of priority when testing a mAb for possible cytotoxicity effects: which methods to use to test for the direct effect (non dependable on CDC/ADCC mechanisms)...

05 May 2013 2,485 3 View