Andrea Tavosanis

3 Questions 6 Answers 0 Followers

Questions related from Andrea Tavosanis

How do you isolate a specific fluorescence signal when some cells also express a reporter gene and some do not?

Hello, I am trying to assess the rate of protein translation in two different cell samples: a WT and a KO. I am using OP-Puro incorporation as the assay of choice and I am looking at the...

09 October 2020 4,276 2 View

What is the significance of a Ki67-negative, DAPI-high population during FACS cell cycle analysis?

Hello, I want to perform FACS analysis to assess the cell cycle status in specific bone marrow cell populations by looking at the Ki67 against DAPI signal. We have working antibodies and DAPI...

15 February 2019 9,501 3 View

Is EdU and BrdU double staining suitable for FACS analysis?

Hello everyone, I am interested in observing differences in cell cycle kinetics for a certain population of cells across different conditions. I want to use EdU/BrdU double staining to look at...

21 October 2018 9,413 7 View