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I am using 0.1% of polysorbate tween in a ion exchange column and I would like to load the eluate into a hydrophobic resin such as a phenyl sephoarose. Does anyone have experience with polysorbate...
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I have been trying to purify a protein through cation exchange chromatography on a Nuvia Q column (BioRad). For some reason when I measure the protein content (through ELISA) in the flow throgh,...
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