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I have been expressing an intrinsically disordered protein (IDP) and have been having issues purifying it. The protein is overexpressed in T7 promoter E.coli cells. The protein has a 6His tag and...
20 February 2018 3,649 6 View
Why is it recommended an OD600 of 0.6-0.8 for protein expression? My E.coli cells have a maximum OD600 of 2.2 and when I induced them at a high OD600 (around 1) the protein was expressed in high...
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Hi there, I am new to Bioinformatics and I am working on a project that requires me to identify orthologs of ABCC4. I have been using Uniport database, and have been identifying many redundant...
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