Ananta Kumar Das

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Questions related from Ananta Kumar Das

In an article, 0.75 µL of each Forward and Reverse Primer (0.2 µM) in qPCR reaction is used. How 0.2 µM is calculated?

In our experiment: Lyophilized Oligo Primers supplied on 50 nmol scale were diluted in 500 µL of 0.1X TE buffer to make it 100 µM stock tube. [(50 nmol/500 µL TE)×(1000 pmol/nmol)=100 pmol/...

01 January 1970 718 6 View

May an index on Bilateral Apex Pubis (BAP) distance have practical implication in poultry breeding?

Could Bilateral Apex Pubis (BAP) distance be a novel index for follicular development and egg laying status in domestic hens (Gallus gallus domesticus)?

01 January 1970 3,814 3 View

Could the phenol:chloroform extraction procedure be an appropriate DNA isolation method for sequencing and fragment analysis?

It was one query of reviewer.

01 January 1970 4,234 2 View

Whether the Classification and regression tree (CRT) be preferable than Artificial neural network (ANN) to determine body weight from age?

When body weight being response variable while ages being independent variable.

01 January 1970 3,500 1 View

What is the basis to equalize the concentration of first strand cDNA to 25ng/μl in each sample for qRT-PCR experiment?

My explanation was as follows: 1 μl cDNA template is used in 20 μl reaction mixture subjected to qRT-PCR experiment, for which template concentration must be strictly equal in each sample under...

01 January 1970 471 2 View

What is the basis to equalize the concentration of first strand cDNA in each sample for qRT-PCR experiment?

In qRT-PCR experiment, we first optimize the concentration of first strand cDNA to 25ng/μl in each sample.

01 January 1970 1,406 5 View

Anyone can explain statistically how square root of sample numbers comes in the computational formula of standard error?

Computational formula of Standard Error = Std. Dev./SQRT (N).

01 January 1970 6,781 2 View