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Questions related from Ana Palacios Ochoa
I want to evaluate the polarization to M2 phenotype in Raw Cells, I co-cultured the cell with LPS and my drug to see the effect of the drug to inhibit the proinflammatory effect of LPS, but I have...
06 May 2024 8,474 0 View
Hi, I want to evaluate stress oxidative markers in macrophages using western blot. I want to know an easy protocol to isolate macrophages from PBMCs, I already know a protocol to isolate them...
25 August 2023 1,474 2 View
I want to study the expression of some proteins related to oxidative stress with western blot. I wonder what microglia cell line is the best for it.
21 August 2023 431 3 View
When I'm running SDS-PAGE 12%, my sample moves to the other well (it's formed as a small curve with the following well) , even if I put it slowly,carefully, 15ul per well and I'm using a 1mm...
03 July 2023 3,321 3 View
I'm using a molecular weight size marker (cat #161-0373), but I don't have all the bands and lately the last band I get was not straight. I run in a 12% gel, I use 80v for the stacking and 200v...
03 July 2023 1,055 0 View
Hi, I want to evaluate oxidative stress in a neuroblastoma cell line, I know about SOD1, but I think it's not enough, so I was investigating and I found 4-hydroxynonenal, I have this in my lab...
28 June 2023 9,063 3 View
Hi, I have been having some problems with SH-SY5Y. I want to differentiate them and do immunofluorescence. However I have had some problems with their growth. They were growing normal (first...
01 January 1970 3,124 4 View
