How many mice do I need to do qPCR of T cells sorted from B16 TILs?

I am trying to analyze the expression of a few molecules in both T CD4+ and T CD8+ lymphocytes from my B16 melanoma tumors (of B6 mice). The issue is that I can't let the tumors grow over 500mm³,...

04 February 2020 9,409 2 View