Amy Replogle

4 Questions 11 Answers 0 Followers

Questions related from Amy Replogle

Did anyone attend Gobal Engage’s qPCR & Digital PCR Congress USA in San Diego last year? Was it worth it? Would you go again?

I am potentially interested in attending Gobal Engage’s 2nd qPCR & Digital PCR Congress USA on July 11-12, 2016 in Philadelphia (http://www.globalengage.co.uk/digital-and-qpcr.html), but I...

01 January 2016 8,747 0 View

Does anyone have any recommendations for housekeeping genes to use in qPCR for rat skeletal muscle?

I have a student who is comparing exercising rats (that had voluntary access to running wheels for 8 weeks), sedentary rats (that were in wheel-free cages for 8 weeks), and de-trained rats (that...

03 March 2015 9,848 4 View

How do I properly normalize T-DNA insertion lines with reference genes?

If I'm doing a qPCR experiment where I need to determine the relative decrease in gene expression from RNAi lines that were generated by T-DNA insertion do I need to run my reference primers on...

02 February 2015 511 3 View

What is the preferred method for determining primer efficiencies for different biological replicates?

If I am doing a qPCR experiment looking at gene expression between a wild-type and mutant and I plan on doing 3 biological replicates what is the best way to factor in primer efficiency? Would you...

05 May 2014 3,498 2 View