Amy Koshoffer

3 Questions 9 Answers 0 Followers

Questions related from Amy Koshoffer

I am having trouble with ECL detection using a VersaDoc system. Any suggestions? I was wondering what ECL detection reagents others are using.

I am running 30 ug-40ug proteins and looking at several proteins ranging from 85kDa to 25 kDa. We have been using a wet transfer system with a good assessed transfer by amido black staining. The...

08 August 2013 9,643 10 View

What keeps epon sections on copper grids?

We are staining EM grids with Lead Citrate and Uranyl acetate. In the most recent prep the lead citrate labeling seems to release the sections from the grid. Can anyone explain why this might...

03 March 2013 5,802 30 View

Can you stain tissue to determine if it was viable at the time of fixation?

I know you can label for apoptosis markers to check if cells in tissue are dying, but what would you recommend to show that tissue is viable outside of morphology.

03 March 2013 1,210 6 View