Amruta Mhashilkar

9 Questions 11 Answers 0 Followers

Questions related from Amruta Mhashilkar

How does one determine therapeutic potential of a nuclear hormone receptor?

When you are investigating a particular NHR signaling pathway for the worm development, how do you know whether it has therapeutic potential or not? Is the treatment likely toxic or not? Can you...

11 July 2014 3,047 1 View

Why do I get an amplification curve in negative samples without template? Can anyone list methods to detect contamination of kit or RNA or reagents?

I am using RNA extracted from whole adult female Brugia malayi worms for qPCR studies. I have been using iTaq Universal SYBR green RT kit for my qPCR. From the first run I am getting a separate...

23 May 2014 4,709 3 View

Should I consider a spliced version of the gene to build the qPCR primers or an unspliced version which has an intron?

I ran an RNAseq with Brugia malayi worms treated with a drug and untreated. I found a certain number of genes differentially expressed in the treated worms as compared to the untreated. I selected...

18 April 2014 8,557 6 View

Can anyone suggest a Ammonium sulphate cut protocol?

My protein is 43kDa. I want to use ammonium sulphate cut to precipate my protein. Can anyone suggest at what concentration saturation will I achieve precipitation of my protein? Further down the...

23 August 2013 5,151 9 View

Can anybody answer a question regarding protein purification?

I am purifying a his-tagged protein. My vector has the his tag and a V5 epitope. I am using anti-V5 antibody for my western. On the western blot, I see a band at the correct molecular weight, but...

11 June 2013 3,417 14 View

Problems with protein expression in bacterial and yeast system

I have cloned my insert using Gateway cloning into a his tagged vectors. Then I transformed BL-21 cells once I confirmed my insert. I grew cells with correct selectable marker. I induced the cells...

05 June 2013 2,313 8 View

I need a software or a bioinformatics tool to find a palindrome sequence in my parasite genome.

I have a palindromic sequence that I need to find in Brugia malayi genome. It is only 13 nucleotides. I tried using BLASTn but it failed the character requirement. I need to find it upstream of...

31 May 2013 1,593 3 View

How to distinguish between expression of an overexpressed protein and an endogenous protein?

I have a cell line that expresses an endogenous protein. Now, if I transfect overexpression in the same protein, how do I know that the results I am getting are due to the overexpression protein?

24 April 2013 1,768 7 View

How to determine whether overexpression of Molecule A alters endogenous Molecule B expression?

My cell line has endogenous expression of Mol.A and B. But, when I overexpress A, I want to know whether it is altering endogenous expression of B or not. What experiment should I conduct to...

24 April 2013 3,864 4 View