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Questions related from Ammon Thompson
I need to see if a gene has higher expression in one tissue type compared to another. I would prefer not to use housekeeping genes for normalization.
09 September 2012 1,649 27 View
All the different sizes of oligo dT seem to have really low Tm (32-39 C) and I've been running my RT reactions at 50 degrees which is around 15 degrees above their Tm values. Is the length of the...
04 April 2012 7,143 5 View
I ran the negative control RT reaction (no RT enzyme) using random hexamers and oligo dT primers. I then used that same sample for qPCR of three different genes in fish skeletal muscle. What is...
03 March 2012 3,516 16 View