Amlan Chakraborty

6 Questions 12 Answers 0 Followers

Questions related from Amlan Chakraborty

What is the best way to block mouse MHC class II for in-vitro cultures?

I intend to block the MHC-II(I-A/I-E) on mouse antigen presenting cells in-vitro. Which technique will be the best?

06 June 2017 2,653 3 View

Why are cells shifting while acquisition after PMT voltage is set with unstained cells?

Hi Everyone, I'm using LSRII for Flow. I have a macrophage population(F4/80+) cells. When I setup the experiment, the PMT voltages of FSC and SSC are perfect with all the cells showing. After...

04 April 2017 2,542 3 View

Is my gene amplifying?

I have a gene(wild type) cloned in pBAD18 Kanamycin resistant plasmid. The gene is 2185 bp. And the plasmid is 5437 bp. I wanted to insert a 6X His tag at the C-terminal end of the protein of this...

03 March 2015 1,027 12 View

What particle can be less than 1 nanometre?

We have synthesized carbon based nanoparticles and have found that it has fluorescent property under UV. Using Malvern particle size analyzer we have found the presence of particles of 3 different...

08 August 2014 774 6 View

How to detect bacteria present in human blood directly?

I want to detect bacteria from blood directly. The same has been possible very easily from stool samples but how will it be possible from blood?

07 July 2014 9,405 11 View

How do you tag a mixture of Phytochemicals with 99mTc?

I want to tag a formulation made of multiple phytochemicals which constitutes flavonoids and alkaloids with 99mTc. I will be using the 99mTc tagged formulation for gamma imaging on mice or New...

07 July 2014 6,986 2 View