Amit Kumar Verma

4 Questions 9 Answers 0 Followers

Questions related from Amit Kumar Verma

Can anyone please share the protocol for electrolyte assay standardized for yeast?

Can anyone please share the protocol for electrolyte assay standardized for yeast? I don't have any equipment for measuring the conductivity, can it be measured using the pH meter?

01 January 2020 6,366 1 View

Problem: Failure to bind the glutathione-Sepharose?

My protein tagged with GST is not binding the glutathione beads whereas free GST is binding very well. I have been purifying the same protein earlier and have got good elutions. I also have tried...

09 September 2013 6,574 1 View

Can polyclonal antibodies crossreact against tagged proteins?

I have cloned my protein into pET32 a + vector which contains thioredoxin tag, His tag and S-tag. I want to use this protein for polyclonal antibody generation in rabbits. Can polyclonal...

08 August 2013 9,718 16 View

Problem with protein expression

Hello everyone, I've cloned an ORF in the pET28a+ and tried to express the protein in E coli BL-21and other strains such as Rosetta, C43 etc. This is a yeast mitochondrial Hsp40 protein. I tried...

01 January 1970 8,838 10 View