Amit Chougule

3 Questions 11 Answers 0 Followers

Questions related from Amit Chougule

How to calculate significance in Seahorse - Mito Fuel Flex assay?

Hi there! I used Agilent's Seahorse - Mito Fuel Flex kit for few experiments. Wave software is quite useful to analyze the data. It gives dependency, capacity and standard deviation values in...

25 November 2019 2,869 1 View

Did anyone had a problem (tissue damage) while performing immunohistochemistry for mouse bone samples?

Hello everyone, I am attempting to stain the trabecular bone of proximal tibia and cortical bone of the midshaft tibia. Tissue was fixed in formalin for approx. 4 months followed by...

07 March 2018 2,522 4 View

Can anyone help me with the deplastinization protocol?

I am working on plastinated bone samples. After performing immuno-histochemistry (IHC) I found staining was okay but specimens were slightly damaged may be because of harsh deplastinizing process.

13 February 2017 6,770 3 View