Amira Ali

5 Questions 1 Answers 0 Followers

Questions related from Amira Ali

I am confused why I have POOR repeatability with ion trap mass spectrometery for the same injection concentration?

Hello, I am developing a quantitative method using HPLC-ESI-MS (ion trap mass spectrometry) in the negative ionization mode. I face an issue but I cannot tell why it is happening. I inject the...

08 August 2024 8,285 0 View

How to activate Dowex50 X8 - Ionic form H+?

Hello, I need to use this ion exchange resin to test the efficacy of the purification step by adding it to a microcon (Blaajberg et al. 2010). This article did not mention the working protocol for...

21 May 2024 8,546 0 View

Why it is not possible to fragment phytic acid (IP6) in the ms2 by HPLC-ESI-MS/MS ion trap but it was fine to fragment others e.g. IP5, IP4, IP3?

I am working on a class of phosphate-containing compounds that is widely present in plants called "Phytates" - This class contains a range of compounds starting from IP6 to IP1 - the numbers here...

10 November 2023 5,943 2 View

Phytate tune method: Fragmention of phytate in -ve ionization mode in mass spectrometry shows no signal, however the 659 m/z was detected, why?

I am working on developing a method for phytate analysis (IP6) using the mass spectrometry detector by tune method to detect the parent ion and precursor ions. The following concentrations of 50,...

01 February 2023 4,081 0 View

Phytates standard curve using UV-Vis, what could be the reason that we get no difference in the absorbances between the 0 & higest concentrations?

I am working on total phytate quantification using the UV-Vis spectrophotometer, and we prepared the phytate standard solutions (0-200 μg/ml phytic acid in deionized water). There was no...

25 November 2022 9,448 2 View