Amber Petersen

3 Questions 7 Answers 0 Followers

Questions related from Amber Petersen

I am expressing SEP-tagged AMPAR subunits but the expression is homogenous throughout the cell, not enriched at the plasma membrane. Any suggestions?

I've been expressing the SEP-tagged GluA1 and GluA2 in organotypic hippocampal slices but am not seeing anything resembling surface AMPARs with either subunit. The GFP signal is homogenous...

29 June 2015 8,076 1 View

How long is the media for organotypic slices usable?

We use hippocampal organotypic slices (prepared P5-6) for electrophysiology and other applications. In our lab it has been customary to prepare slice culture media, aliquot and store at 4C for up...

03 January 2014 7,526 4 View

Recording resting membrane potential

I am trying to record resting membrane potential from hippocampal pyramidal neurons in whole-cell current-clamp mode. The problem I've been having is that the RMP observed is much less negative...

06 January 2013 2,656 45 View