Amandine Bordat

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Questions related from Amandine Bordat

Do someone have a idea about avoiding hairpin formation of fragments during Gibson assembly reaction (single strand phase)?

I have some problem with Gibson assembly reaction. I work on viruses, and virus genome do some secondary structures and hairpin sometimes. As we have a only reaction at 50°C for Gibson assembly, I...

02 August 2017 6,176 25 View

Minus 80 to minus 70°C - leaves sample integrity?

Dear everbody, We planed to put our minus 80°C freezer to minus 70°C (economy!!). But we have questions about the integrity of our plant tissue samples (especially infected ones). We have found...

01 January 1970 8,916 0 View