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Questions related from Amanda Vazquez
I’m determining esterase activity with pNPB as substrate, using Tris-HCl buffer. In optimal pH assays (ranging from 2-12), I’m observing substrate hydrolysis in pH 7 and higher; the intensity of...
02 February 2018 3,259 4 View
Hi, we are thinking about PEGylating a protein, for which dimerization or oligomerization seems to be important in its activity. Could this be affected by attaching PEG? Thanks.
01 January 2017 3,571 6 View
Hello. We are going to use the 5 Prime cell-free expression system in our lab, to obtain a recombinant peptide of around 5 kDa. In most references is mentioned that the expression is successful...
01 January 2017 2,245 5 View
Hello. I´m trying to grow yeasts (S. cerevisiae and C. albicans) in both YEPD and Sabouraud agar, but I don´t see any colonies even after 48 h of incubation at 28 or 37ºC. What could be happening?...
11 November 2016 5,998 10 View
The fusion protein I´m working with stains well with coomassie, but not with silver. After the fusion hydrolysis, the digestion subproducts are stained both with silver and coomassie, but I can´t...
09 September 2016 8,882 4 View
