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Questions related from Amanda Lovecraft
I was wondering someone might have any idea what could cause the result we observe below. We are studying binding between a protein and small molecule ligand. In order to determine the enthalpy...
05 May 2019 3,184 3 View
I spotted aqueous farnesyl pryophosphate ammonia salt on PEI cellulose TLC plate and then stained with iodine. About 1-2 hours after removing it from the iodine chamber, the outer ring, where the...
11 November 2016 228 3 View
I would like to determine if my compound of interest, farnesyl pyrophosphate, is degrading in our storage conditions. My plan is to run PEI-TLC using solvent isopropanol:ammonia:water, 6:3:1, by...
10 October 2016 2,862 3 View
I have a plasmid map and a plasmid which I've sequenced, and I found a discrepancy where the map contains two copies of TetO separated by ~30bp whereas my sequencing result shows only one copy of...
04 April 2016 3,428 0 View
I'm familiar with the lac operon, but I'm looking at plasmid maps and seeing various annotations that confuse me. Does anyone have a guide to these? Why does it say PLlacO instead of pLacO? Extra...
04 April 2015 4,384 1 View
I'm performing FACS on e coli, but the cells are clumping together so each event is multiple cells.E EDIT: I determined the problem. Bacteria must be gated on SSC threshold, NOT FSC threshold as...
01 January 2015 812 4 View
I am setting up a competition assay where I'll be competing E coli strains against each other. Each strain is genetically marked with a fluorescent protein, e.g. CFP and YFP. Can anyone recommend...
10 October 2014 3,345 4 View
